Jumping into Metastable 1:1 Urea–Succinic Acid Cocrystal Zone by Freeze-Drying

Preservation of Lactic Acid Bacteria by Freeze-Drying

Lactic acid bacteria are widely used in the manufacture of fermented milk products, such as cheeses, sour cream butter, yoghurt and fermented milk beverages. These microorganisms may also be applied to the production of fermented meat products and silage. We may easily cultivate lactic acid bacteria in milk. However, it is a laborious work to preserve the organisms in liquid state because viabi...

Preservation of enteroviruses by freeze-drying.

A method was developed for freeze-dry stabilization of poliovirus type 3. An ultrafiltration procedure was used to remove salts from infected tissue culture fluid, and the virus was freeze-dried after suspension in an alkaline organic buffer. This method was further tested with other picornaviruses including poliovirus types 1 and 2, coxsackieviruses A9, A20, B2, and B5, echovirus 11, and the e...

Freeze-drying of bioproducts: putting principles into practice.

The product and process parameters that determine successful freeze-drying are described and their interrelationships are explored. It is shown that the thermochemical and thermomechanical properties of water-soluble, amorphous materials form the basis of effective formulation design and that coordinated approaches to formulation and process development achieve optimum results with a minimum of...

Freeze-drying of bioproducts: putting principles into practice

Circulating-Gas Freeze Drying

WAGMAN, JACK (U.S. Army Biological Laboratories, Frederick, Md.) AND EDWARD J. WENECK. Preservation of bacteria by circulating-gas freeze drying. Appl. Microbiol. 11:244-248. 1963.-Water-washed Serratia marcescens and Escherichia coli were freeze dried in a circulating-gas system at atmospheric pressure. This convective procedure resulted in a substantially higher survival of organisms than cou...